Coding

Part:BBa_K4835035:Design

Designed by: Kun Zhang   Group: iGEM23_XJU-China   (2023-10-11)


Enhancing the speed and accuracy of cell translation in high saline environments.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 340
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 340
    Illegal BglII site found at 354
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 340
    Illegal NgoMIV site found at 1101
    Illegal AgeI site found at 379
    Illegal AgeI site found at 589
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 550


Design Notes

If you want to use it in a project, you need to be careful not to affect other genes.


Source

Reference Genome: Enterobacter cloacae RS35


References